Modeling of the macromolecular composition dynamics of microalgae batch culture

Lelekov A.S., Trenkenshu R.P.
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The work focuses on mathematical modeling of light influence mechanisms on macromolecular composition of microalgae batch culture. It is shown that even with a single limiting factor, the growth of microalgae is associated with a significant change in the biochemical composition of the biomass in any part of the batch curve. The well-known qualitative models of microalgae are based on concepts of enzymatic kinetics and do not take into account the possible change of the limiting factor during batch culture growth. Such models do not allow describing the dynamics of the relative content of biochemical components of cells. We proposed an alternative approach which is based on generally accepted two-stage photoautotrophic growth of microalgae. Microalgae biomass can be considered as the sum of two macromolecular components — structural and reserve. At the first stage, during photosynthesis a reserve part of biomass is formed, from which the biosynthesis of cell structures occurs at the second stage. Model also assumes the proportionality of all biomass structural components which greatly simplifies mathematical calculations and experimental data fitting. The proposed mathematical model is represented by a system of two differential equations describing the synthesis of reserve biomass compounds at the expense of light and biosynthesis of structural components from reserve ones. The model takes into account that a part of the reserve compounds is spent on replenishing the pool of macroergs. The rates of synthesis of structural and reserve forms of biomass are given by linear splines. Such approach allows us to mathematically describe the change in the limiting factor with an increase in the biomass of the enrichment culture of microalgae. It is shown that under light limitation conditions the batch curve must be divided into several areas: unlimited growth, low cell concentration and optically dense culture. The analytical solutions of the basic system of equations describing the dynamics of macromolecular biomass content made it possible to determine species-specific coefficients for various light conditions. The model was verified on the experimental data of biomass growth and dynamics of chlorophyll $a$ content of the red marine microalgae Pоrphуridium purpurеum batch culture.

Keywords: mathematical model, growth rates, biochemical composition, light intensity, linear splines, light absorption coefficient, chlorophyll $a$
Citation in English: Lelekov A.S., Trenkenshu R.P. Modeling of the macromolecular composition dynamics of microalgae batch culture // Computer Research and Modeling, 2023, vol. 15, no. 3, pp. 739-756
Citation in English: Lelekov A.S., Trenkenshu R.P. Modeling of the macromolecular composition dynamics of microalgae batch culture // Computer Research and Modeling, 2023, vol. 15, no. 3, pp. 739-756
DOI: 10.20537/2076-7633-2023-15-3-739-756
URL: http://crm-en.ics.org.ru/journal/article/3342/
Creative Commons License This work is licensed under a Creative Commons Attribution-NoDerivs 3.0 Unported License.

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