DNA conformational dynamics: approach to the physical mapping of genome

Nechipurenko Y.D., Nechipurenko D.Y., Il’icheva I.A., Golovkin M.V., Panchenko L.A., Polozov R.V., Grokhovsky S.L.
 pdf (388K)  / List of references

Recently we have developed a new method for studying DNA based on ultrasound - induced cleavage of DNA sugar-phosphate backbone. Relative cleavage rates of the phosphodiester bonds in all 16 dinucleotides have been determined. The increased amount of data sampling (of more than 20 000 nucleotides) made it also possible to obtain cleavage rates in all 256 possible tetranucleotides. These values quantitatively characterize sequence effects on conformational dynamics of DNA sugar phosphate backbone. Same type of DNA heterogeneity have been discovered and studied using its chemical cleavage induced by various chemical agents and DNAse I. The presence of essential heterogeneity in structural properties of DNA might be a key for physical mapping of the genomes, i.e. determining the structural profiles being responsible for DNA recognition by gene expression regulation machinery.

Keywords: ultrasonic cleavage of DNA, sequence effects on DNA structure, DNA flexibility, d(CpG) dinucleotide
Citation in English: Nechipurenko Y.D., Nechipurenko D.Y., Il’icheva I.A., Golovkin M.V., Panchenko L.A., Polozov R.V., Grokhovsky S.L. DNA conformational dynamics: approach to the physical mapping of genome // Computer Research and Modeling, 2010, vol. 2, no. 4, pp. 419-428
Citation in English: Nechipurenko Y.D., Nechipurenko D.Y., Il’icheva I.A., Golovkin M.V., Panchenko L.A., Polozov R.V., Grokhovsky S.L. DNA conformational dynamics: approach to the physical mapping of genome // Computer Research and Modeling, 2010, vol. 2, no. 4, pp. 419-428
DOI: 10.20537/2076-7633-2010-2-4-419-428
URL: http://crm-en.ics.org.ru/journal/article/1752/
Creative Commons License This work is licensed under a Creative Commons Attribution-NoDerivs 3.0 Unported License.
According to Crossref, this article is cited by:
  • I.N. Vasilyeva, V.N. Zinkin. The value of low-molecular-weight DNA of blood plasma in the diagnostic of the patological processes of different genesis. // Biomeditsinskaya Khimiya. — 2013. — V. 59, no. 3. — P. 358. DOI: 10.18097/pbmc20135903358
Please note that citation information may be incomplete as it includes data from Crossref cited-by program partners only.
Views (last year): 2. Citations: 2 (RSCI).

Indexed in Scopus

Full-text version of the journal is also available on the web site of the scientific electronic library eLIBRARY.RU

The journal is included in the Russian Science Citation Index

The journal is included in the RSCI

International Interdisciplinary Conference "Mathematics. Computing. Education"

Copyright © 2009–2024 Institute of Computer Science