Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates

Ozerov I.V., Osipov A.N.
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Here we demonstrate the results of kinetic modeilng of DNA double-strand breaks induction and repair and phosphorilated histone H2AX ($\gamma$-H2AX) and Rad51 foci formation in primary human fibroblasts exposed to low-LET ionizing radiation (IR). The model describes two major paths of DNA double-strand breaks repair: non-homologous end joining (NHEJ) and homologous recombination (HR) and considers interactions between DNA and several repair proteins (DNA-PKcs, ATM, Ku70/80, XRCC1, XRCC4, Rad51, RPA, etc.) using mass action equations and Michaelis–Menten kinetics. Experimental data on DNA rejoining kinetics and $\gamma$-H2AX and Rad51 foci formation in vicinity of double strand breaks in primary human fibroblasts exposed to low-LET IR with various dose rates and exposure times was utilized for training and statistical validation of the model.

Keywords: DNA double-strand breaks, DNA repair, kinetic modeling, low-LET ionizing radiation, dose rate
Citation in English: Ozerov I.V., Osipov A.N. Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates // Computer Research and Modeling, 2015, vol. 7, no. 1, pp. 159-176
Citation in English: Ozerov I.V., Osipov A.N. Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates // Computer Research and Modeling, 2015, vol. 7, no. 1, pp. 159-176
DOI: 10.20537/2076-7633-2015-7-1-159-176
URL: http://crm-en.ics.org.ru/journal/article/2237/
Creative Commons License This work is licensed under a Creative Commons Attribution-NoDerivs 3.0 Unported License.
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