Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates

 pdf (2412K)  / List of references

Here we demonstrate the results of kinetic modeilng of DNA double-strand breaks induction and repair and phosphorilated histone H2AX ($\gamma$-H2AX) and Rad51 foci formation in primary human fibroblasts exposed to low-LET ionizing radiation (IR). The model describes two major paths of DNA double-strand breaks repair: non-homologous end joining (NHEJ) and homologous recombination (HR) and considers interactions between DNA and several repair proteins (DNA-PKcs, ATM, Ku70/80, XRCC1, XRCC4, Rad51, RPA, etc.) using mass action equations and Michaelis–Menten kinetics. Experimental data on DNA rejoining kinetics and $\gamma$-H2AX and Rad51 foci formation in vicinity of double strand breaks in primary human fibroblasts exposed to low-LET IR with various dose rates and exposure times was utilized for training and statistical validation of the model.

Keywords: DNA double-strand breaks, DNA repair, kinetic modeling, low-LET ionizing radiation, dose rate
Citation in English: Ozerov I.V., Osipov A.N. Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates // Computer Research and Modeling, 2015, vol. 7, no. 1, pp. 159-176
Citation in English: Ozerov I.V., Osipov A.N. Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates // Computer Research and Modeling, 2015, vol. 7, no. 1, pp. 159-176
DOI: 10.20537/2076-7633-2015-7-1-159-176
Views (last year): 4. Citations: 3 (RSCI).

Full-text version of the journal is also available on the web site of the scientific electronic library eLIBRARY.RU

The journal is included in the Russian Science Citation Index

The journal is included in the List of Russian peer-reviewed journals publishing the main research results of PhD and doctoral dissertations.

International Interdisciplinary Conference "Mathematics. Computing. Education"

The journal is included in the RSCI

Indexed in Scopus