Kinetic modeling of radiation-induced DNA double-strand break repair incorporating an ultraslow component

Корнева С.А., Chernyaev A.P., Osipov A.N.
 pdf (480K)

A kinetic model of radiation-induced DNA double-strand break repair with an ultraslow component of complex damage is proposed. The model was parameterized using experimental time courses of $\gamma$H2AX and pATM foci in human mesenchymal stem cells after exposure to $\gamma$-rays (60Co) and 14.1 MeV neutrons at a dose of 0.5 Gy. The standard two-component scheme reproduced the early response phase (0.5–6 h) but systematically underestimated the residual marker signal at 24 h, especially after neutron irradiation. Introducing an ultraslow component allowed both the early and late phases of the response to be described without fitting separate kinetic parameters for different radiation types. The differences between γ-irradiation and neutrons were introduced only through the distribution of damage between fractions, including fixed ultraslow fractions $\pi g = 0.15$ and $\pi n = 0.40$. Model comparison using $\chi^2$ and AIC, together with bootstrap analysis and local sensitivity assessment, supports the robustness of the conclusion that an ultraslow damage fraction is required to describe late post-radiation signaling.

Keywords: DNA double-strand breaks, DNA repair, kinetic modeling, ultraslow damage fraction, linear energy transfer (LET), neutron irradiation, DNA damage markers
Citation in English: Корнева С.А., Chernyaev A.P., Osipov A.N. Kinetic modeling of radiation-induced DNA double-strand break repair incorporating an ultraslow component // Computer Research and Modeling, 2026, vol. 18, no. 4, pp. 989-1003
Citation in English: Корнева С.А., Chernyaev A.P., Osipov A.N. Kinetic modeling of radiation-induced DNA double-strand break repair incorporating an ultraslow component // Computer Research and Modeling, 2026, vol. 18, no. 4, pp. 989-1003
DOI: 10.20537/2076-7633-2026-18-4-989-1003
URL: http://crm-en.ics.org.ru/journal/article/3755/
Creative Commons License This work is licensed under a Creative Commons Attribution-NoDerivs 3.0 Unported License.

Copyright © 2026 Корнева С.А., Chernyaev A.P., Osipov A.N.

Indexed in Scopus

Full-text version of the journal is also available on the web site of the scientific electronic library eLIBRARY.RU

The journal is included in the Russian Science Citation Index

The journal is included in the RSCI

International Interdisciplinary Conference "Mathematics. Computing. Education"

Website Copyright © 2009–2026 Institute of Computer Science